In Figure 4, the sequence GGTCAA was typed in the Find Sequence window and the Return key pressed. Another way to find a specific base in FinchTV is to enter a sequence that is located near or contains your base. Further sequence analysis revealed the presence of a Leu-Asp-Val (LDV) motif in the HEV penton base amino acid sequence similar to most of the human adenoviruses. In addition, the index hopping rate in DNBSEQ TM platforms is much lower as compared to that of other platforms. Experiment by selecting a base number in your sequence. The HEV penton base lacks the RGD motif, present in most human adenoviruses (Ad2, Ad3, Ad4, and Ad 12) suggesting that HEV may not use alpha v integrins to gain entry into host cells. When combined with the PCR-free library construction method, DNBSEQ TM also has higher SNP and indel detection accuracy compared to other platforms. These advantages dramatically improve sequencing accuracy, and have much lower duplication rates in WGS/WES applications. cPAS technology has been widely used on various sequencing platforms including BGISEQ-50, BGISEQ-500, DNBSEQ-G50, DNBSEQ-G400, DNBSEQ-T7, etc.Ĭompared to other existing sequencing platforms, DNBSEQ TM sequencing technology combines the advantages from low amplification error rates from DNBs and high density patterned arrays. It includes: DNA single strand circularization and DNB preparation technology, Patterned Arrays, DNB loading, cPAS (combinatorial Probe Anchor Synthesis), Pair-End Sequencing technology on DNBs, fluidics and imaging systems, base calling algorithms, etc. This information is then used to determine the DNA sequence of the sample.Īll sequencing technologies relating to DNBs are part of DNBSEQ TM. The images are then converted into a digital signal using MGI's propriety software. Images are taken after the fluorescently labeled probes on the DNB are excited with lasers. The sequencing reaction starts by pumping sequencing reagents containing fluorescently labeled dNTP probes and DNA polymerase. Sequencing primer is then added and hybridized to the adaptor region of the DNB. DNBs (DNA nanoballs) are pumped with by the fluidics system and loaded onto a Patterned Array chip. MGI's DNA sequencing instruments utilize the state-of-the-art core technology called DNBSEQ TM.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |