Eine sehr frühe Gabe des damals üblichen Doldenhopfens sollte eine Verbesserung der Ausbeute an Bitterstoffen bewirken. Conscious of the impacts, this study helps interpret the minimized fermentation and sheds light on the development of MTP based HTS platform for anaerobic cultivations.Įrste Erwähnungen der Vorderwürzehopfung finden sich Ende des 19. These influences were partly attributed to their impacts on air accessibility. Additionally, fermentative activities were reduced by excluding air in either the material or the headspace of MTP. The air-tight MTP cover, as compared to the gas-permeable cover, not only balanced the above volatile flavors but also maintained integrity to the endogenous carbon dioxide pressure during beer fermentation. More than 1.5-folds acetaldehyde and higher alcohols, yet less than half esters, were obtained from the MTP and SF fermentations relative to that in the EBC tube. The shaking MTP did not promote early growth but sustained significantly higher cell numbers at the later fermentation stage. The downscaling from 2 mL to 0.2 mL in MTP increased the cell growth rate and vitality but did not change the maximum cell density. The microscale beer fermentation was closer to the SF but differed greatly from EBC tube fermentation depending on the MTP configurations. The lager strains in MTPs accelerated cells reproduction and vitalization, sugar consumption, and glycerol accumulation. pastorianus beer fermentation compared to that in the shaking flask (SF) and European Brewing Convention (EBC) tube regarding fermentation performances and flavor attributes. This study investigates the impacts of MTP configurations (scale, shaking, and cover) on the S. ![]() Downscaling the anaerobic fermentation in a microtiter plate (MTP) facilitates high throughput screening (HTS) applications.
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